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KMID : 0921620080380020053
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Journal of Bacteriology and Virology
2008 Volume.38 No. 2 p.53 ~ p.60
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Proteomic analysis of Helicobacter pylori J99 Outer Membrance Protein by Tandem Mass Spectrometry
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Kim Kyung-Mi
Kang Hyung-Lyun
Cho Myung-Je
Youn Hee-Shang
Lee Seung-Gyu
Joo Jung-Soo
Baik Seung-Chul
Lee Woo-Kon
Rhee Kwang-Ho
Kwon Young-Chul
Bea Dong-Won
Song Jea-Young
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Abstract
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The protein identity of sarcosine-insoluble outer membrane proteins (OMPs) of Helicobacter pylori J99 was determined with the basic study of understanding the function of proteins. A sarcosine-insoluble OMPs was resolved by two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were shown in the alkaline pI regions (6.0~11.0) with molecular masses of 10 to 100 kDa. We have performed an extensive proteome analysis by quadrupole time of flight (Q-TOF) mass spectrometry (MS). Here, of 50 spots processed, 42 spots were identified, which represented 16 genes and we newly detected 8 kinds of proteins (JHP0119, JHP0388, JHP1046, JHP1405, JHP0073, JHP0551, JHP1382, JHP0552) from the sarcosin-insoluble fraction of H. pylori J99. Those may be used to elucidate the characterization of the OMPs of H. pylori J99, which will help identify new potential target proteins for vaccine development and drug therapy.
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KEYWORD
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Helicobacter pylori, Outer Membrance Protein, Q-TOF MS
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